2E48
Crystal Structure of Human D-Amino Acid Oxidase: Substrate-Free Holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-24 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 149.652, 181.727, 50.815 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.21284 |
Rwork | 0.210 |
R-free | 0.25929 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2du8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.347 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.102 | 0.245 |
Number of reflections | 31102 | |
<I/σ(I)> | 5.9 | |
Completeness [%] | 97.3 | 82.9 |
Redundancy | 3.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG 4000, 0.1M sodium citrate, 0.2M ammonium acetate, 10% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |