2E42
Crystal structure of C/EBPbeta Bzip homodimer V285A mutant bound to A High Affinity DNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-02 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.881, 113.319, 74.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.420 - 1.800 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gu4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | |
| Number of reflections | 39915 | |
| Completeness [%] | 98.8 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | magnesium chloride, MES, PEG400, PH 6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | magnesium chloride | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | PEG400 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | magnesium chloride | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | PEG400 | ||
| 8 | 1 | 2 | H2O |
