2E3S
Crystal structure of CERT START domain co-crystallized with C24-ceramide (P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.243, 75.646, 42.327 |
| Unit cell angles | 90.00, 108.44, 90.00 |
Refinement procedure
| Resolution | 40.160 - 1.940 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Number of reflections | 17894 | |
| Completeness [%] | 98.1 | 83.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP |
