2E3Q

Crystal structure of CERT START domain in complex with C18-ceramide (P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	53.196, 54.783, 90.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.830 - 2.080
R-factor	0.184
Rwork	0.182
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.564
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.080	2.080
Number of reflections	16553
Completeness [%]	99.9	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				VAPOR DIFFUSION, HANGING DROP