2E3B

Crystal structure of the HA-bound form of Arthromyces ramosus peroxidase at 1.3 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-19
Detector	MARRESEARCH
Wavelength(s)	0.71
Spacegroup name	P 42 21 2
Unit cell lengths	73.150, 73.150, 114.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.300
R-factor	0.1484
Rwork	0.141
R-free	0.19870
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	0.031
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	SHELX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	45.620	1.370
High resolution limit [Å]	1.300	4.110	1.300
Rmerge	0.068	0.047	0.279
Total number of observations		37068	69481
Number of reflections	78980
<I/σ(I)>	2.6	11.3	0.8
Completeness [%]	99.4	99.7	97.4
Redundancy	8	13.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.5	297	37% saturated ammonium sulfate, Tris-HCl, pH 7.5, MICRODIALYSIS, temperature 297K