2E2J
RNA polymerase II elongation complex in 5 mM Mg+2 with GMPCPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Collection date | 2005-11-01 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.142, 222.017, 194.652 |
| Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.500 |
| R-factor | 0.24401 |
| Rwork | 0.242 |
| R-free | 0.30602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.492 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 84357 | |
| <I/σ(I)> | 10.2 | 1.3 |
| Completeness [%] | 95.4 | 81.3 |
| Redundancy | 8.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 9-11% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2HPO4 | ||
| 2 | 1 | 1 | NaH2PO4 | ||
| 3 | 1 | 1 | dioxane | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | PEG6000 | ||
| 6 | 1 | 2 | (NH4)2HPO4 | ||
| 7 | 1 | 2 | NaH2PO4 | ||
| 8 | 1 | 2 | PEG6000 |
