2E2E
TPR domain of NrfG mediates the complex formation between heme lyase and formate-dependent nitrite reductase in Escherichia Coli O157:H7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Detector technology | CCD |
Collection date | 2005-05-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97950, 0.97956, 0.97171 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.802, 85.824, 49.073 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.050 |
R-factor | 0.23499 |
Rwork | 0.231 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.433 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 22119 | |
Completeness [%] | 98.7 | 95.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | ||||
1 | ||||
1 |