2E22
Crystal structure of xanthan lyase in complex with mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2005-12-20 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.358, 91.462, 159.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.150 - 2.400 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.064 | 0.254 |
| Number of reflections | 29738 | |
| Completeness [%] | 88.6 | 42.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4000, 0.2M ammonium formate, 0.1M sodium Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
