2E1A
crystal structure of FFRP-DM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.800, 95.800, 95.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.000 - 2.500 |
| Rwork | 0.190 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z4p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.299 |
| Number of reflections | 35886 | |
| <I/σ(I)> | 7.7 | 2.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 278 | 1.0M LiCl, 10% PEG 6000, 100mM citrate, 20% glycerol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
