2E11
The Crystal Structure of XC1258 from Xanthomonas campestris: A CN-hydrolase Superfamily Protein with an Arsenic Adduct in the Active Site
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97905, 0.96108 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 143.280, 154.305, 51.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 104.830 - 1.730 |
R-factor | 0.18636 |
Rwork | 0.140 |
R-free | 0.21746 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.345 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.830 | 104.830 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.140 | 0.140 |
Number of reflections | 112277 | |
<I/σ(I)> | 9 | 9.1 |
Completeness [%] | 98.4 | 95.3 |
Redundancy | 5.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |