2E0Z
Crystal structure of virus-like particle from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-11 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 631.500, 631.500, 351.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.600 |
Rwork | 0.268 |
R-free | 0.26700 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.132 | 0.844 |
Number of reflections | 773334 | |
<I/σ(I)> | 8.8 | 1.6 |
Completeness [%] | 96.8 | 97.5 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 16~20% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP | |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 16~20% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP |