2E0Q
Crystal structure of K53E thioredoxin from Sulfolobus tokodaii strain7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.951, 52.123, 33.794 |
Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.490 |
R-factor | 0.17294 |
Rwork | 0.172 |
R-free | 0.19185 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.490 |
Number of reflections | 57836 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 16% PEG-3000, 0.1M acetate (pH 4.1), 25% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |