2DZY

Crystal structure of N392A mutant of yeast bleomycin hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	95
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 63 2 2
Unit cell lengths	151.027, 151.027, 89.669
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.290 - 2.570
R-factor	0.202
Rwork	0.198
R-free	0.25300
Structure solution method	isomorphous replacement
Starting model (for MR)	1gcb
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.570
Number of reflections	19700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	14-20% PEG 4K,100mM Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K