2DZE
Crystal structure of histone chaperone Asf1 in complex with a C-terminus of histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.155, 52.130, 60.654 |
| Unit cell angles | 107.35, 105.94, 93.95 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.21514 |
| Rwork | 0.214 |
| R-free | 0.24352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cu9 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.191 |
| Number of reflections | 38738 | |
| Completeness [%] | 94.1 | 91.4 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG6000, AS, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
