2DYO

The crystal structure of Saccharomyces cerevisiae Atg5- Atg16(1-57) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 42 21 2
Unit cell lengths	73.253, 73.253, 148.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.730 - 1.970
R-factor	0.215
Rwork	0.215
R-free	0.24500
Structure solution method	MIR, MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.055	0.304
Number of reflections	29390
<I/σ(I)>	28.9
Completeness [%]	99.9	100
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	15% PEG 3350, 0.1M HEPES, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K