2DYL
Crystal structure of human mitogen-activated protein kinase kinase 7 activated mutant (S287D, T291D)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.552, 69.635, 84.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.120 - 2.450 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.28260 |
Structure solution method | MIR |
Starting model (for MR) | 1s9j |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 13708 | |
<I/σ(I)> | 26.0099 | 2.95349 |
Completeness [%] | 99.0 | 97 |
Redundancy | 5.77064 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 300 | 0.1M Tris, 0.2M ammonium acetate, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |