2DYJ
Crystal structure of ribosome-binding factor A from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-05-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.426, 65.838, 43.173 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 42.920 - 1.840 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jos |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 13813 | |
| <I/σ(I)> | 24.4 | 3.22 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.73 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2M Ammonium dihydrogen phosphate, 0.08M Tris-HCl pH8.5, 9.6% v/v Glycerol anhydrous, 0.02M Bis-Tris propane pH 7.0, 0.08M Magnesium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
