2DYC
Crystal structure of the N-terminal domain of mouse galectin-4
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-07 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9790, 0.9794, 0.970 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 92.053, 92.053, 132.908 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.500 - 2.400 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 11598 | |
<I/σ(I)> | 17.2 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.4M Ammonium Sulfate, 12% Glycerol, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |