2DWK

Crystal structure of the RUN domain of mouse Rap2 interacting protein x

Replaces:  1WUS

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-11-27
Detector	RIGAKU
Wavelength(s)	0.9792, 0.9795, 0.9640
Spacegroup name	P 64 2 2
Unit cell lengths	86.523, 86.523, 106.582
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.590 - 2.000
R-factor	0.205
Rwork	0.205
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	16483
<I/σ(I)>	14.5	5.2
Completeness [%]	99.6	100
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	300	0.05M SODIUM CHLORIDE, 1.4M AMMONIUM SULFATE, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K