2DWJ
Structure of the complex of C-terminal lobe of bovine lactoferrin with raffinose at 2.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.670, 50.430, 65.950 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 17.460 - 2.300 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b17 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.460 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16836 | |
| Completeness [%] | 94.0 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES, PEG MONOMETHYLETHER, 0.1M ZNSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
