2DVY
Crystal structure of restriction endonucleases PabI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.570, 113.956, 89.173 |
Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.31800 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.400 | 3.000 |
Rmerge | 0.067 | 0.035 | 0.283 |
Number of reflections | 30368 | ||
<I/σ(I)> | 14.8 | ||
Completeness [%] | 99.3 | 99.8 | 96.7 |
Redundancy | 3.6 | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 5% PEG 6000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |