2DVQ
Crystal structure analysis of the N-terminal bromodomain of human BRD2 complexed with acetylated histone H4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-07-07 |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.811, 55.143, 67.489 |
| Unit cell angles | 90.00, 94.06, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.040 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BD1 NATIVE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.068 | 0.281 |
| Number of reflections | 25699 | |
| Completeness [%] | 96.1 | 78.1 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, Co-crystallizaation | 6.5 | 298 | PEGMME 5K, AS, pH 6.50, VAPOR DIFFUSION, Co-crystallizaation, temperature 298K |
