2DV6
Crystal structure of nitrite reductase from Hyphomicrobium denitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-19 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 |
Unit cell lengths | 162.551, 162.551, 148.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.15233 |
Rwork | 0.152 |
R-free | 0.16530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kbw |
RMSD bond length | 0.017 |
RMSD bond angle | 1.839 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.590 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 195178 | |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | PEG4000, potassium thiocyanate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |