2DUE

crystal structure of a green fluorescent protein variant S65T/H148D at pH 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	50.887, 62.590, 69.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.240
R-factor	0.1547
Rwork	0.148
R-free	0.20400
Structure solution method	AB INITIO
RMSD bond length	0.013
RMSD bond angle	0.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.280
High resolution limit [Å]	1.240	1.240
Number of reflections	59945
Completeness [%]	93.7	61.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	298	100mM MgCl2, 100mM CHES pH 10, 26% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K