2DTS
Crystal Structure of the Defucosylated Fc Fragment from Human Immunoglobulin G1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32B2 |
Synchrotron site | SPring-8 |
Beamline | BL32B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-07 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.472, 77.690, 143.462 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.19483 |
Rwork | 0.192 |
R-free | 0.25064 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h3x |
RMSD bond length | 0.033 |
RMSD bond angle | 2.593 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.669 |
Number of reflections | 28426 | |
<I/σ(I)> | 10.2 | 2.6 |
Completeness [%] | 98.9 | 99.7 |
Redundancy | 4.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7 | 277 | Deionized water, pH 7.0, MICRODIALYSIS, temperature 277K |