2DRU

Crystal structure and binding properties of the CD2 and CD244 (2B4) binding protein, CD48

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Collection date	1999-02-16
Wavelength(s)	0.977
Spacegroup name	I 41 2 2
Unit cell lengths	96.780, 96.780, 126.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.200 - 2.600
R-factor	0.214
Rwork	0.214
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ccz
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.600
Number of reflections	9426
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M sodium HEPES, 1.5M lithium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K