2DRR
Crystal structure of reducing-end-xylose releasing exo-oligoxylanase D263N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.182, 86.235, 87.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.230 - 1.600 |
| R-factor | 0.1627 |
| Rwork | 0.162 |
| R-free | 0.18164 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1wu4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.157 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.430 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 53617 | |
| <I/σ(I)> | 27.9 | 3.1 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG4000, SODIUM ACETATE, GLYCEROL, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
