2DQX
mutant beta-amylase (W55R) from soy bean
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.835 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.042, 85.042, 145.083 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wdp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | |
| Number of reflections | 31087 | |
| Completeness [%] | 98.9 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 0.1M sodium acetate (pH 5.4), 1mM EDTA, 50% ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
