2DQM
Crystal Structure of Aminopeptidase N complexed with bestatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.417, 120.417, 170.859 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
Rwork | 0.182 |
R-free | 0.19400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2dq6 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.280 |
Data reduction software | MOSFLM |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 187171 | |
<I/σ(I)> | 18.8 | 3.9 |
Completeness [%] | 99.4 | 96.8 |
Redundancy | 6.4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | the crystal is soaked in 2.2M ammonium sulfate, 0.1M MES pH 6.4, 1mM bestatin. , VAPOR DIFFUSION, HANGING DROP, temperature 293K |