2DQ7

Crystal Structure of Fyn kinase domain complexed with staurosporine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	52.962, 52.962, 210.989
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
Rwork	0.255
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qpc
RMSD bond length	0.008
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	9281
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	1.15M ammonium sulfate, 0.2M NaCl, 0.1M imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K