2DPI
Ternary complex of hPoli with DNA and dCTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.148, 98.148, 203.112 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.640 - 2.300 |
| R-factor | 0.23209 |
| Rwork | 0.228 |
| R-free | 0.27818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2alz |
| RMSD bond length | 0.046 |
| RMSD bond angle | 3.477 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.282 |
| Number of reflections | 26566 | |
| <I/σ(I)> | 49.9 | 10.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 16% PEG 5K MME, 0.4M Ammonium Sulfate, 0.1M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 5K MME | ||
| 2 | 1 | 1 | Ammonium Sulfate | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | PEG 5K MME | ||
| 6 | 1 | 2 | Ammonium Sulfate | ||
| 7 | 1 | 2 | MES | ||
| 8 | 1 | 2 | HOH |
