2DP5
Structure of streptococcus pyogenes bacteriophage-associated hyaluronate lyase Hylp2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 59.587, 59.587, 588.546 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.550 |
| R-factor | 0.22273 |
| Rwork | 0.221 |
| R-free | 0.23625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c3f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.773 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.650 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Number of reflections | 5360 | |
| <I/σ(I)> | 4.7 | 1.9 |
| Completeness [%] | 98.1 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Tris HCl, Sodium formate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
