2DNJ
DNASE I-INDUCED DNA CONFORMATION. 2 ANGSTROMS STRUCTURE OF A DNASE I-OCTAMER COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 273 |
| Detector technology | OSCILLATION CAMERA |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.900, 100.100, 92.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.174 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.031 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.076 * |
| Number of reflections | 20921 |
| Completeness [%] | 89.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6 | 4 * | pH 6.00, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | EDT | ||
| 4 | 1 | 1 | IMIDAZOLE | ||
| 5 | 1 | 1 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | EDTA | 15 (mM) | ||
| 2 | 1 | PEG6000 | 8 (%(w/v)) |
