Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 89.800, 58.800, 102.000 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.18 * |
| Rwork | 0.185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dik |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.790 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.099 * | |
| Total number of observations | 97563 * | |
| Number of reflections | 35455 | |
| Completeness [%] | 95.0 | 83 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 30 * | pH 7.0, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | imidazole | 20 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | EDTA | 0.1 (mM) | |
| 7 | 1 | drop | mercaptoethanol | 1 (mM) |
