2DHR
Whole cytosolic region of ATP-dependent metalloprotease FtsH (G399L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 146.153, 146.153, 349.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.900 |
| R-factor | 0.301 |
| Rwork | 0.299 |
| R-free | 0.34200 |
| Structure solution method | MIR |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.100 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.077 | 0.547 |
| Number of reflections | 40304 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M Tris-HCl, 1% PEG8K, 0.2mM Zn acetate, 2.5mM ADP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
