2DH5
Crystal structure of E. coli Holo-TrpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-26 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 172.516, 172.516, 82.587 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.330 - 2.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN B of 1BKS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.400 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.076 | 0.390 |
| Number of reflections | 15920 | |
| Completeness [%] | 96.8 | 97.4 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro batch using paraffin oil | 8 | 288 | ammonium sulfate, pH 8.0, micro batch using paraffin oil, temperature 288K |
