2DH3
Crystal Structure of human ED-4F2hc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.497, 101.786, 121.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.21972 |
| Rwork | 0.217 |
| R-free | 0.27508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.186 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.650 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 19650 | |
| <I/σ(I)> | 15.74 | 3.46 |
| Completeness [%] | 94.0 | 96.2 |
| Redundancy | 7.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 25% PEG 3350, 0,2M AMMONIUM SULFATE, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
