2DH1
Crystal structure of peanut lectin lactose-azobenzene-4,4'-dicarboxylic acid-lactose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 |
Unit cell lengths | 92.750, 92.750, 473.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 7.650 |
R-factor | 0.35562 |
Rwork | 0.355 |
R-free | 0.37720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pel |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 7.800 |
High resolution limit [Å] | 7.650 | 7.650 |
Rmerge | 0.100 | 0.490 |
Number of reflections | 2273 | |
<I/σ(I)> | 4.6 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.1 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 8000, 0.05M sodium phosphate, 0.2M sodium cholride, 0.02% sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |