2DGN
Mouse Muscle Adenylosuccinate Synthetase partially ligated complex with GTP, 2'-deoxy-IMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2002-03-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.94 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.112, 70.112, 199.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.198 |
| R-free | 0.26240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.415 |
| Number of reflections | 20145 | |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 98.9 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 12%-14% PEG 8K, 200mM Magnesium Acetate, pH 7.0, temperature 298K |
