2DGD
Crystal structure of ST0656, a function unknown protein from Sulfolobus tokodaii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9792, 0.9795, 0.9685 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 131.221, 131.221, 125.896 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.990 - 2.900 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.22200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 31282 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 9.3 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 0.6M NaCl, 20% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |