2DG2
Crystal Structure of Mouse Apolipoprotein A-I Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97898 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.855, 125.745, 163.620 |
| Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
| Resolution | 80.000 - 2.450 |
| R-factor | 0.20724 |
| Rwork | 0.206 |
| R-free | 0.22844 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.729 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.500 |
| Rmerge | 0.083 | 0.509 |
| Number of reflections | 69794 | |
| <I/σ(I)> | 22.4 | 2.4 |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.5M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
