2DFL

Crystal structure of left-handed RadA filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 43
Unit cell lengths	55.183, 55.183, 125.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.226
Rwork	0.217
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t4g
RMSD bond length	0.011
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.880	2.880
Rmerge	0.053	0.723
Number of reflections	8521
<I/σ(I)>	27.8	2.1
Completeness [%]	99.8	100
Redundancy	8.3	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	3% PEG4000, 50mM Na-acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4	298	3% PEG4000, 50mM Na-acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K