2DEX
Crystal structure of human peptidylarginine deiminase 4 in complex with histone H3 N-terminal peptide including Arg17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.072, 60.103, 115.703 |
| Unit cell angles | 90.00, 124.32, 90.00 |
Refinement procedure
| Resolution | 33.130 - 2.100 |
| R-factor | 0.20698 |
| Rwork | 0.203 |
| R-free | 0.24698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wda |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.130 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 46064 | |
| Completeness [%] | 94.6 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Imidazole (pH8.0), 0.2M lithium sulfate, 10% PEGMME2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
