2DEG
Crystal structure of tt0972 protein form Thermus Thermophilus with Mn2(+) ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-19 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.324, 65.324, 202.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.23845 |
Rwork | 0.236 |
R-free | 0.27870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cz8 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.542 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.044 | 0.399 |
Number of reflections | 49302 | |
<I/σ(I)> | 33.1 | 4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% MPD, 200mM ammonium acetate, 100mM lithium chloride, 10mM manganese chloride, 100mM citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |