2DEC
Crystal Structure of the PH0510 protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.228, 79.485, 67.497 |
| Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j5x |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.091 | 0.344 |
| Number of reflections | 68213 | |
| <I/σ(I)> | 6.9 | 2.81 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.8 | 295 | PEG 3350, lithium acetate, pH 7.8, microbatch, temperature 295K |
