2DCK
A tetragonal-lattice structure of alkaliphilic XynJ from Bacillus sp. 41M-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 |
| Unit cell lengths | 116.579, 116.579, 45.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.16812 |
| Rwork | 0.167 |
| R-free | 0.19799 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dcj |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.985 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.520 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 36572 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10mM sodium phosphate buffer (Na2HPO4/NaH2PO4), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
