2DBU
Crystal Structure of Gamma-glutamyltranspeptidase from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97902, 0.97931, 0.98214 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.700, 126.900, 128.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.390 - 1.950 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.059 | 0.369 |
Number of reflections | 94722 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.2 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 15% PEG 4000, 0.2M magnesium sulfate, 5% glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |