2DBO

Crystal structure of D-Tyr-tRNA(Tyr) deacylase from Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	76.010, 76.010, 133.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.010 - 2.760
Rwork	0.230
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7g
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.010	2.860
High resolution limit [Å]	2.760	2.760
Number of reflections	6373
<I/σ(I)>	28.8534	7.72
Completeness [%]	100.0	100
Redundancy	12.3	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	8	277	0.15M sodium chloride, pH 8.0, SMALL TUBES, temperature 277K