2DB4
Crystal structure of rotor ring with DCCD of the V- ATPase from Enterococcus hirae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.214, 125.229, 211.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 107.830 - 2.400 |
| R-factor | 0.22529 |
| Rwork | 0.225 |
| R-free | 0.23094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.830 | 2.462 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.091 | 0.605 |
| Number of reflections | 124723 | |
| <I/σ(I)> | 11.2 | 1.8 |
| Redundancy | 4.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 32% PEG 400, 240mM Sodium citrate, 100mM Tris-HCl (pH 7.5), 4% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
