2D98
Structure of VIL (extra KI/I2 added)-xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-03 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.191, 38.564, 56.794 |
Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
Resolution | 20.100 - 2.000 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.25300 |
Structure solution method | MIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.300 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 10211 | |
Completeness [%] | 91.1 | 80.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | 1.5M ammonium sulfate, 0.1M Na citrate , pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |